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. 2014 May 8;6(3):e00143. doi: 10.1042/AN20130034

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Co-localization of GFAP (red) and BrdU (green) after CCI or sham injury around the cortical lesion and in the corpus callosum at different time points. Proliferating astrocytes have the morphology of reactive astrocytes with hypertrophic cell body and processes. Arrows indicate cells shown at larger magnification in their corresponding panels. Some sections taken at 3 dpi were triple labeled for GFAP⁺/S100β⁺/BrdU⁺ (E1-4) confirming that dividing GFAP⁺ cells were astrocytes. Scale bar=50 μm (A, B, C and D) and 20 μm (a, b, c, d and E). (F) Quantification of GFAP⁺/BrdU⁺ cells at different time points after CCI or sham injury (mean±S.E.M., n=3–6). *P<0.05, **P<0.01, by two-tailed Student's t test comparing sham and CCI at each time point.