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. 2014 May 8;6(3):e00143. doi: 10.1042/AN20130034

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Confocal images show localization of proliferating NG2⁺ cells (NG2⁺, red; BrdU⁺, green) around the cortical site of injury at 1, 3 and 7 dpi. Proliferation of these cells was highest at 1 (A, a) and 3 dpi (B, b), although it was also high in sham brains (D, d). Proliferation of NG2⁺ cells decreased by 7 dpi (C, c). Arrows indicate cells shown at larger magnification in their corresponding panels. Scale bar=50 μm (A, B, C and D) and 20 μm (a, b, c and d). (E) Quantification of NG2⁺/BrdU⁺ cells at different time points after CCI or sham injury (mean±S.E.M., n=3–6). *P<0.05, by two-tailed Student's t test comparing sham and CCI at each time point.