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. 2014 May 8;6(3):e00143. doi: 10.1042/AN20130034

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Confocal images show colocalization of both DCX⁺ (red) and BrdU⁺ (green) cells in the corpus callosum (cc) (A), in addition to the SVZ (B) at 1 dpi. DCX+/BrdU+ cells are seen in cortical areas at 3 dpi (C, cell indicated by arrow is shown in higher magnification in D). We observed no colocalization of BrdU and DCX at 7 dpi in the cortex (E); note the presence of clusters (or chains) of DCX⁺ cells (F) at 7 dpi. Scale bar=50 μm (A, C and E) and 20 μm (B, D and F). (G) Quantification of DCX⁺/BrdU⁺ cells after CCI or sham injury (mean±S.E.M., n=3–6). *P<0.05, by two-tailed Student's t test comparing sham and CCI at 3 dpi.