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. 2014 May 8;6(3):e00144. doi: 10.1042/AN20130045

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) C6 glioma cells were activated for different times, as indicated, in presence of LI. (B) C6 glioma cells were pre-incubated for different times, as indicated in presence of LI and at the end of the pre-incubation time, cells were washed three times with PBS and the medium was replaced by fresh plain medium for the subsequent 24 h. NO production was assessed indirectly by measurement of nitrites (the Griess method). Data are expressed as means±S.E.M. (n=6). Results were analyzed by two-way ANOVA followed by the Bonferroni's post hoc test. ***P<0.001 versus control.