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. 2014 May 8;6(3):e00144. doi: 10.1042/AN20130045

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Total cytosolic RNA was prepared from the control, or microglial cells treated with C-CM or LI-CM for different times, and used for real-time Q-PCR analysis of different inflammatory genes, (A) CD86, (B) TNFα, (C) IL-1β, (D) TGFβ, (E) arginase I,ARG and (F) IL-10. Data are expressed as fold change versus control, taken as calibrator for comparative quantitation analysis of mRNA levels. Each sample was measured in triplicate, the experiment was repeated two times with similar results. Data are means±S.E.M., and were analyzed by two-way ANOVA followed by the Bonferroni's post hoc test. ***, P<0.001, **P<0.01, *P<0.05 versus control; °°°P< 0.001, °P<0.05 versus C-CM.