Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Oct 22.

Published in final edited form as: ACS Nano. 2013 Sep 26;7(10):8605–8615. doi: 10.1021/nn403311c

(a) Fluorescence from TOPRO3 binding to siRNA increases dramatically at pH ≤ 5.5 when packaged via p5RHH ( ), but not the non-functioning peptide p5RWR ( ). (b) Polyacrylamide gel electrophoresis confirms that p5RHH releases siRNA at pH 4.5 but p5RWR shows no pH-dependent release. (c) p5RHH is also released at low pH with an increase in p5RHH release at pH ≤ 5.5. (d) Freed p5RHH is capable of hemolysis, leading to increased hemoglobin release at pH ≤ 5.5. (e–h) Acridine orange release assays show that p5RHH/siRNA nanoparticles are able to disrupt endosomes (h) when tested in tissue culture, as exhibited by dye release similar to that of 100μM chloroquine (f), whereas p5RWR cannot (g). Scale bar 50μm.

Inline graphic — (a) Fluorescence from TOPRO3 binding to siRNA increases dramatically at pH ≤ 5.5 when packaged via p5RHH ( ), but not the non-functioning peptide p5RWR ( ). (b) Polyacrylamide gel electrophoresis confirms that p5RHH releases siRNA at pH 4.5 but p5RWR shows no pH-dependent release. (c) p5RHH is also released at low pH with an increase in p5RHH release at pH ≤ 5.5. (d) Freed p5RHH is capable of hemolysis, leading to increased hemoglobin release at pH ≤ 5.5. (e–h) Acridine orange release assays show that p5RHH/siRNA nanoparticles are able to disrupt endosomes (h) when tested in tissue culture, as exhibited by dye release similar to that of 100μM chloroquine (f), whereas p5RWR cannot (g). Scale bar 50μm.