FIG. 2.

Effect of PRKA stimulators and hormones on strontium-induced activation. Treatment with AICAR (A), AMP (B), or RSVA405 (C) during oocyte maturation (prematuration) prevented strontium-induced activation in both CEO and DO, but supplementation after the 17-h maturation period (postmaturation) was relatively ineffective. D) Amphiregulin (AR) or FSH suppressed strontium-induced activation in CEO when present during the initial maturation period. E) Effect of delaying AICAR treatment on strontium-induced activation. The inhibitory effect of AICAR on activation in CEO was decreased when AICAR treatment was initiated 8 h but not 6 h after the start of culture. All data are presented as mean percent activated ± SEM. Groups with no common letter are significantly different (CEO, a–d; DO, w–z).