FIG. 3.

β-galactosidase activity and ATP levels in Eno4^Gt/Gt and WT testes. A) β-galactosidase activity was detected in germ cells in testis from Eno4^Gt/Gt mice by X-gal staining. B) No β-galactosidase activity was detected in testis from WT mice by X-gal staining. Bars = 25 μm. C) Enolase activity. Sperm were suspended in an imidazole buffer containing 2 mM MgSO₄ and 50 mM imidazol-HCl (pH 6.8) and sonicated, and total enolase activity was assayed. Enolase activity in sperm from Eno4^Gt/Gt mice was significantly lower than in WT mice (n = 4; *P < 0.05). D) ATP levels. The ATP levels in sperm from Eno4^Gt/Gt mice were significantly lower than in WT mice (n = 3; *P < 0.05).