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Differences in solubility of PGAM2, enolase, ENO4, and AKAP4. Most of the PGAM2 (detected with PGAM antibody) and some of the enolase (detected with pan-enolase antibody) was detected in the 1% NP-40 sperm lysate. The majority of the enolase was solubilized by 6 M urea. ENO4 and AKAP4 were present in the 6 M urea insoluble sperm fraction.
