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. 2013 Feb 27;88(4):90. doi: 10.1095/biolreprod.112.107128

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© 2013 by the Society for the Study of Reproduction, Inc.

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FIG. 9 — Coimmunoprecipitation assay with ENO4, AKAP4, ENO, and PGAM2. A) Regions of ENO4_V1 (aa 1–571), truncated N-terminal ENO4-N (aa 1–228), and ENO4-C (aa 228–618) used for in vitro synthesized [³⁵S]-labeled probes. B) [³⁵S]-ENO4_V1 and [³⁵S]-ENO4-C were coimmunoprecipitated from sperm lysates with AKAP4 by the antiserum to AKAP4 but not by rabbit IgG. C) [³⁵S]-ENO4-C was coimmunoprecipitated from sperm lysates with ENO1 or GM5506 by the antibody to pan-enolase but not by rabbit IgG. D) [³⁵S]-PGAM2 was coimmunoprecipitated from sperm lysates with ENO1 or GM5506 by the antibody to pan-enolase but not by rabbit IgG.