FIG. 2.

A) [1-¹³C]-glucose consumption by 129 and B6 WT sperm measured by NMR spectroscopy. At time 0, [1-¹³C]-glucose was added to 15 × 10⁶ living sperm and utilization of [1-¹³C]-glucose was monitored every hour for 20 h. Columns correspond to the calculated nanomoles of glucose consumed per hour per 10⁶ sperm. B) Activity of different glycolytic enzymes was measured in 129 WT and B6 WT sperm extracts (HK, hexokinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PK, pyruvate kinase; LDH, lactate dehydrogenase). Values are the mean ± SEM for 129 versus B6, n = 3 minimum, *P < 0.01.