FIG. 5.

In situ hybridization of Rhox8 in mouse ovary. Representative photomicrographs show that Rhox8 mRNA is reduced in granulosa cells of preantral follicles in Rhox5-null mice (A, B) eCG primed, 2 h post-hCG but not altered in large antral follicles (C, D) eCG primed, 6 h post-hCG. Arrows show accumulation of Rhox8 antisense probe in some oocytes that is not translated (Fig. 2C) or may be nonspecific. E) No appreciable signal was observed with the Rhox8 sense strand probe in any tissue section.