Table 1. Primary antibodies used in the present study.
Antibody against | Clone number | Host | Pretreatment | Dilution | Source |
---|---|---|---|---|---|
Collagen type I | COL-1 | Mouse | 0.1% Trypsin, 5 min | 1:1,000 | A |
Collagen type III | FH-7A | Mouse | 0.1% Trypsin, 5 min | 1:2,000 | A |
Tenascin-C | 578 | Rat | Citric acid buffer, microwave, 5 min | 1:50 | B |
COMP | MA37C94 | Rat | 0.1% Trypsin, 5 min | 1:20 | C |
A: Sigma-Aldrich (Sigma, St. Louis, MO, USA), B: RD Systems (Minneapolis, MN, USA), C: Serotec (Oxford, UK).