Table 2. Sequences of the RT-PCR primer sets used in this study.
| Primer | Sequence | Product size (bp) |
|---|---|---|
| Collagen I alpha I | 5’-ACCTGCATACAGGACGGCCTC-3’
(Forward) 5’-GCCAGGCACGGAAATTCCAGC-3’ (Reverse) |
420 |
| Collagen III alpha I | 5’-CACAAAGAGTCTCATGTCTGA-3’
(Forward) 5’-GGTCACCATTTCTCCCAGGA-3’ (Reverse) |
397 |
| Tenascin-C | 5’-GGACAATGAGATGCGGGTCAC-3’
(Forward) 5’-GTCTGTGACATCTTTCACCTC-3’ (Reverse) |
520 |
| COMP | 5’-GACAGTCGGGACAACTGCCG-3’
(Forward) 5’-CCAGCTCAGGGCCCTCATAG-3’ (Reverse) |
682 |
| GAPDH | 5’-GACCACAGTCCATGCCATCAC-3’
(Forward) 5’-GTCCACCACCCTGTTGCTGTA-3’ (Reverse) |
454 |