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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: FEBS J. 2014 Apr 7;281(9):2266–2283. doi: 10.1111/febs.12780

Figure 3. L-FABP Tyrosine fluorescence in denaturation conditions.

Figure 3

Tyrosine fluorescence spectra of rat, Human T94T WT and T94A variant in 8M urea (Panel A) and 6M Guanidinium chloride (Panel B) were recorded as described in Experimental Procedures (solid line, rat L-FABP; dotted line, T94T; dash line; T94A). Panel C, normalized difference spectra of (normalized rat L-FABP - normalized T94T) tyrosine fluorescence emission (solid line, buffer; ---, 8M urea; -··-, 6M GnHCl). Representative spectra were shown.