Figure 4. ANS binding to Rat, T94T, T94A L-FABPs.
Panel A, representative ANS (35μM) emission spectra when bound to L-FABPs (500nM). Panel B, representative reverse titration curves. ANS (100nM) were titrated with increasing amount of the L-FABP. Panel C, representative forward titration curves. L-FABP 500nM were titrated with increasing amount of ANS. Solid line, buffer only, no proteins; ●, rat L-FABP; ○, T94T; ▼, T94A.