Figure 5. ANS displacement from L-FABP by long chain fatty acids (LCFAs).
L-FABP (500nM) was incubated with ANS 35μM for 10min. ANS displacement were done by titration with small aliquots of ligands (Panel A, palmitic acid; Panel B, stearic acid; Panel C, oleic acid; Panel D, linoleic acid; Panel E, arachidonic acid; Panel F, eicosapentaenoic acid; Panel G, docosahexaenoic acid.) ●, rat L-FABP; ○, T94T; ▼, T94A.