FIGURE 1. GPR17 Deficiency Increases CysLT1R-mediated Df-induced Pulmonary Inflammation.
(A) Inflammatory cell counts in BAL fluid. For active sensitization and challenge, mice received 1 μg of Df (black columns) or PBS (white columns) by intranasal injection twice per wk for 3 wks, and BAL was performed 2 d after the last injection. Total and differential cell counts for monocytes/macrophages (MΦs), neutrophils, eosinophils, and lymphocytes are shown. Values are the mean ± SEM (n = 8-10) combined from 3 independent experiments. *P < 0.01, #P < 0.05. (B) Histologic analyses of the lung. After BAL, lung tissues were fixed with paraformaldehyde and stained with H&E. b, bronchi, v, vessels. Immunohistochemistry was performed with mouse mAb to α-smooth muscle actin (SMA) and ABC-alkaline phosphatase reagent, visualized in red. Scale bars = 100 μm for HE, 50 μm for SMA.