FIGURE 4. GPR17 Deficiency Upregulates Df-induced CysLT₁R Expression in Inflammatory Cells in Lungs.

Immunohistochemistry for CysLT₁R in lungs of WT, Cysltr1^−/−, Gpr17^−/−, and Gpr17/Cysltr1^−/− mice sensitized and challenged with PBS or Df. Lungs were stained with polyclonal rabbit anti-CysLT₁R IgG and developed with ABC-peroxidase reagent visualized in brown. Scale bar = 50 μm. Data are representative of results from 2 of the experiments depicted in Figure 1. Magnified pictures of representative macrophages (MΦ), PMNs, eosinophils (Eos), and lymphocytes (Lym) from Df-challenged groups are shown in lower panels.