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Comparison of NCS-related LeuA88 and LeuB88 of PDB entry 1jbb refined with and without torsion NCS restraints. (a) Refinement without NCS restraints allows the incorrectly built LeuB88 side chain to remain a tp rotamer while distorting the surrounding backbone geometry. (b) Refinement using torsion NCS restraints preserves similar backbone geometry, which causes the incorrectly built LeuB88 side chain to present as an outlier. Images were generated using KiNG (Chen et al., 2009 ▶).
