Table 3. Coefficient of Variation for the Lysosomal Assays.
| original reagent | new reagent | |||
|---|---|---|---|---|
| intra-assay CV (%)a | interassay CV (%)b | intra-assay CV (%) | interassay CV (%) | |
| MPS-I | 6.74 | 9.41 | 1.93 | 1.71 |
| MPS-II | 1.90 | 5.56 | 8.47 | 8.65 |
| MPS-VI (anion exch., EtOAc) | 6.45 | 8.31 | 2.49 | 2.36 |
| MPS-VI (EtOAc) | 4.94 | 5.30 | 3.28 | 5.15 |
a
Intra-assay CV was calculated from triplicate injections of an identical sample into the mass spectrometer.
b
Interasssay CV is calculated from three independent assays carried out with three different punches of the same dried blood spot.