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Quantification of 30S r-proteins (A) and 50S r-proteins (B) by LC–MSE as described by Silva et al.8 Ribosomal proteins are not uniformly distributed and are not detected in one copy per ribosome as determined by LC–MSE. Amounts of small subunit and large subunit proteins are normalized to r-protein S4 and r-protein L3, respectively. Error bars represent the standard deviation of three replicate measurements.
