Figure 5.

Loss of PARK9 enhances zinc-mediated α-Syn accumulation and reduction of lysosomal hydrolases activities. (A) Upper left: western blot analysis of the effect of Zn²⁺ on α-Syn expression in PCNs treated with Scrb or PARK9 shRNA. NSE was used as loading control. MW is shown as kDa. Bottom left: the results of densitometric analysis of α-Syn oligomers which were detected with syn 505 (*P < 0.01). Upper right: the results of densitometric analysis of α-Syn monomers which were detected with C-20 (*P < 0.01). (B) Glucocerebrosidase (GCase) activities in lysosome-enriched fractions extracted from PCNs (n = 3, *P < 0.005). (C) Acid sphingomyelinase (aSMase) activities in lysosome-enriched fractions extracted from PCNs (n = 3, *P < 0.03).