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. 2014 Jan 12;23(11):2981–2994. doi: 10.1093/hmg/ddu009

Figure 8.

Figure 8.

slaks564 encodes Synaptojanin 1 (Synj1). (A and B) Transmission electron microscopy showing normal ribbons in wild-type larvae (arrowheads in A) and floating ribbons in slak mutants at 7 dpf (arrowheads in B). (C) Schematic representation of the splice donor site mutation in slak that causes a frame shift in the coding sequence. The G to A mutation in slak results in a cryptic splice site 14 bp upstream of the original site and produces a premature stop codon. IHC of Synj1 and Cacna1f in slak mutants at 4 dpf. Synj1 is expressed in the OPL and IPL (E and G), whereas Cacna1fa is expressed only in the OPL (F and G) in wild type (WT). In synj1 mutants, Synj1 is absent from both the OPL and IPL (H and J), whereas Cacna1fa is expressed in the OPL (I and J). Scale bar: (A) and (B), 500 nm; (G) and (J), 20 µm.