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The expression of WNT3A proteins in Western blot. WNT3A is detected as an approximately 45-kDa band on Western blots of protein extracted from both the ganglionic and aganglionic colon segment tissue. Immunoblot shows a remarkable WNT3A signal protein in the aganglionic tissues but weak in the ganglionic tissue. Lines 1 and 3: the WNT3A proteins of the aganglionic segment; lines 2 and 4: the WNT3A proteins of the ganglionic segment.
