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. 2014 Mar 15;7(4):1502–1513.

Figure 2.

Figure 2

Effects of Nrf2 knockdown on cisplatin sensitivity in resistant A2780cp cells. A. After cells were transfected with Nrf2 siRNA (100 nM) for 48 h, cell lysates were collected for western blot analyses for Nrf2, Keap1, NQO1 and HO-1. B. Cells were transfected with Nrf2 siRNA (100 nM) for 48 h, and then treated with 40 μg/ml cisplatin for 24 h. Cell viability was determined using a WST-8 assay. Data (mean ± SD) represent the mean value of three independent experiments. **P<0.01. C. Cells were treated with TUNEL and DAPI staining and observed by fluoroscopy (magnification was 20×). D. Cells were subjected to Annexin V and PI staining. The apoptosis ratio is presented as a result of early-stage and advanced stage apoptosis.