Table 2.

Massively parallel pyrosequencing of TET2, CBL and KRAS in fresh mononuclear cell samples and decalcified, formalin-fixed, paraffin-embedded bone marrow trephine biopsies from four CMML patients

		Fresh mononuclear cell sample			dFFPE bone marrow trephine biopsy
	Sequence variation	Allele frequency (%)	Reads	Sanger	Allele frequency (%)	Reads	Sanger
Case 1	TET2 p.Q916X	42.92	890	pos.	45.9	1011	pos.
	TET2 p.I1762V	50.84	2026	pos.	49.13	2019	pos.
	TET2 p.I1873T	42.97	1145	pos.	43.48	1334	pos.
	TET2 p.I1873MfsX14	6.2	1145	neg.	6.6	1334	neg.
Case 2	CBL p.L380P	30.5	2279	pos.	31.6	883	pos.
	TET2 p.Y1255LfsX13	49.1	1564	pos.	37.23	462	pos.
	TET2 p.L1816X	46.5	1688	pos.	41.9	432	pos.
Case 3	CBL p.L380P	51.91	836	pos.	45.71	862	pos.
	TET2 p.G355D	50.68	876	pos.	50.52	1348	pos.
	TET2 p.D527EfsX6	12.78	1221	neg.	11.37	1381	neg.
	TET2 p.D527EfsX6	27.17	530	pos.	30.8	685	pos.
	TET2 p.H1380Y	50.59	597	pos.	42.23	753	pos.
	TET2 p.I1762V	48.84	1116	pos.	43.86	1058	pos.
Case 4	TET2 p.N752KfsX59	34.59	743	pos.	38.51	1688	pos.
	TET2 p.D1704EfsX9	5.19	752	neg.	4.19	1741	neg.
	TET2 p.I1762V	48.94	1561	pos.	51.3	3302	pos.