Table IV. Differentially expressed GC cell envelope proteins identified by iTRAQ.

Accession	Name	Gene	Average ratio ± S.D.a			Sb	Tc
			F62:FA1090	MS11:FA1090	1291:FA1090
Outer membrane
Q5F726	Multidrug efflux pump channel protein	mtrE/NGO1363	1.08 ± 0.04	2.65 ± 0.02	0.93 ± 0.15	Y	N
Q5F6Q4	Transferrin-binding protein A	tbpA/NGO1495	2.93 ± 0.25	0.83 ± 0.09	0.67 ± 0.07	Y	N
Periplasmic
Q5F9E2	Putative type IV pilin-like protein	NGO0456	0.14 ± 0.04	1.11 ± 0.11	0.98 ± 0.05	N	N
Q5F724*	Antibiotic resistance efflux pump component MtrCd	mtrC/NGO1365	0.93 ± 0	2.98 ± 0.39	0.28 ± 0.06	Y	Y
Inner membrane
Q5F725	Antibiotic resistance efflux pump component	mtrD/NGO1364	1.08 ± 0.01	3.21 ± 0.51	0.69 ± 0.02	N	N
Unknown
Q5F5N1	Lipoprotein	NGO1889	2.56 ± 0.09	2.4 ± 0.45	3.35 ± 0.02	N	N
Q5F865	Putative peroxiredoxin family protein/glutaredoxin	NGO0926	0.39 ± 0.1	0.35 ± 0.11	0.27 ± 0.07	N	N
Q5F9E4	Type IV pilus assembly protein PilW	pilW/NGO0454	0.34 ± 0.09	0.24 ± 0.02	0.26 ± 0.06	N	N
Cytoplasmic
Q5F5Q8	Elongation factor Tu	tuf1/NGO1842	0.41 ± 0.1	0.24 ± 0.03	0.3 ± 0.03	N	N
Q5F5T6	30S ribosomal protein S17	rpsQ/NGO1830	0.64 ± 0.23	0.41 ± 0.04	0.45 ± 0.02	N	N
Q5F5V3	50S ribosomal protein L17	rplQ/NGO1817	3.65 ± 0.07	1.7 ± 0.16	1.4 ± 0.05	N	Y

^a Average ratio and standard deviation were calculated for differentially expressed proteins identified in both biological replica experiments with associated p < 0.05.

^b Predictions of signal peptide cleavage site using SignalP server (47).

^c Presence of twin-arginine signal peptide as determined by TatP (48).

^d MtrC protein has been shown to be localized to the periplasm (111).