Fig. 3.

Re-annotation of the transcription termination factor Rho from R. denitificans OCh114. Synthesis of the protein YP_680835.1 was predicted to start from a GTG initiation codon based on homologous sequences found in other members of the Roseobacter clade. However, N-terminal peptides demonstrated that the real start site was 30 nucleotides upstream from the initial annotation (ATG codon, underlined and in bold red). This codon was only conserved in the closest-related strain R. litoralis Och149. Previously annotated codons are shown in red and their counterparts in other sequences are in blue. Acetylated residues are indicated by a star.