Fig. 4.

Multiple translation start sites of the ATP-dependent Clp protease proteolytic subunit. Three alternative start sites were recorded by multiple detected N-terminal peptides (represented as blue lines) for protein YP_683070.1. A multiple sequence alignment with closely-related homologs demonstrates the heterogeneity of initiation sites and how this protein should be re-annotated in multiple species as all three codons are strictly conserved (ATG codons underlined and in bold red). The translated polypeptides from the previous annotation and from the new corrected annotation are labeled 1 and 2, respectively.