Fig. 5.

Re-annotation of the iojap-like protein initiated with a non-conventional start codon. The sequence alignment of the iojap-like protein (YP_681006.1) is shown with closely-related bacteria. The translated polypeptide from the previous annotation and from the new corrected annotation are labeled 1 and 2, respectively. Previously annotated codons are shown in red and their counterparts in other sequences are in blue. The two alternative start codons are indicated in bold red and underlined. Blue lines represent the N-terminal peptide sequences identified in R. denitrificans OCh114. Initiation heterogeneity is demonstrated and re-annotation of this protein in closely-related species is suggested in red.