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. 2013 Feb;32(1):32–35. doi: 10.1089/mab.2012.0069

FIG. 2.

FIG. 2.

(A) Detection of E. coli using MAb 49.4-15. CSFE-labeled E. coli were stained with MAb 49.4-15 followed by incubation with PE-labeled anti-mouse IgG. (a) CSFE-labeled E. coli; (b) MAb 49.4-15 staining of E. coli; (c) merged image. (B) Detection of phagocytized and internalized E. coli using MAb 49.4-15. RAW264.7 cells were incubated with CSFE-labeled E. coli and further stained with MAb 49.4-15 followed by incubation with PE-labeled anti-mouse IgG. (a,e) DAPI staining; (b,f) treatment with CSFE-labeled E. coli alone; (c,g) staining with MAb 49.4-15 followed by staining with PE-labeled anti-mouse IgG; (d,h) merged images; (a–d) horizontal image; (e–h) ortho view of z stack.