Table 1. Comparison of α-L-iduronidase enzyme activity in liver of heterozygous mice from different studies.
IDUA activity (nmol/h/mg) | Final [S] (μM) | Substrate source | Adjustment | Incubation time (hour) | Reaction temperature(°C) | Ref. |
---|---|---|---|---|---|---|
0.8 | 25 | CalBiochem | N | 1 | 22 | [2] |
2 | 25 | CalBiochem | N | 1 | 22 | [3] |
0.3 | 25 | Glycosynth | N | 1 | 37 | [4] |
0.6 | 25 | Glycosynth | N | 1 | 37 | [5] |
0.8 | 25 | Glycosynth | N | 1 | 37 | [6] |
2.5 | 25 | Glycosynth | N | 1 | 37 | [7] |
2.1 | 50 | CalBiochem | N | 1–6 | 37 | [8] |
0.8 | 50 | TRC | N | 6–8 | 37 | [9] |
1.6 | 50 | TRC | N | 6–8 | 37 | [10] |
6 | 50 | CalBiochem | N | 6–8 | 37 | [11] |
6.6 | 1000 | CalBiochem | N | 17 | 37 | [12] |
0.9 | 1425 | CalBiochem | N | 6 | 37 | [13] |
2.8 | 1425 | Glycosynth | N | 6 | 37 | [14] |
6 | 1425 | Glycosynth | N | 6 | 37 | [15] |
4.4 | 180 | Glycosynth | Y | 0.5 | 37 | This study |
N=NO, Y=YES.