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View full-text article in PMC

. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Nat Commun. 2014;5:3318. doi: 10.1038/ncomms4318

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Overexpression of ARPP19 in prophase oocytes has a dual effect on meiotic maturation induced by Pg.

(a) ARPP19 is present in fractions from prophase (Pro) and MII-arrested (MII) oocytes extracted by TCA precipitation and heat treatment. ARPP19 was immunoblotted using a specific ARPP19 antibody.

(b) High amounts of injected WT-GST-ARPP inhibit meiotic maturation. Prophase-arrested oocytes (Pro) were injected with indicated concentrations of WT-GST-ARPP (WT, blue lines). After Pg stimulation, GVBD time course was followed. See also Supplementary Table 1.