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Electrophoresis and size-exclusion chromatography of the SKICH domain of human TAX1BP1. Left, SDS–PAGE of the purified crystallization construct of the SKICH domain. Lanes 1 and 2 contain molecular-weight marker (labeled in kDa) and the SKICH domain preparation, respectively. In the molecular-weight maker, the protein at 14.4 kDa is lysozyme. Right, the SKICH domain is comparable to lysozyme in terms of elution time in size-exclusion chromatography.
