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Purification of recombinant N-acetylmannosamine kinase from S. aureus. Lane 1, molecular-weight markers (labelled in kDa); lane 2, crude cell lysate; lane 3, pooled fractions from anion-exchange chromatography; lane 4, pooled fractions from hydrophobic interaction chromatography; lane 5, pooled fractions from size-exclusion chromatography.
