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Purification of recombinant N-acetylmannosamine-6-phosphate 2-epimerase from methicillin-resistant S. aureus. Lane 1, molecular-weight markers (labelled in kDa); lane 2, crude cell lysate; lane 3, pooled fractions from the anion-exchange chromatography step; lane 4, pooled fractions from the hydrophobic interaction chromatography step; lane 5, pooled fractions from the size-exclusion chromatography step.
