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. 2014 May 8;10(5):e1004310. doi: 10.1371/journal.pgen.1004310

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Induction of the SOS system upon hypoxanthine (Hx) starvation. Cultures of optA1, gpt, and optA1 gpt strains containing a plasmid-carried SOS reporter gene (umuC::lacZ) were grown in minimal medium (with 1% casamino acids) in the absence of Hx for 2, 4, and 6 hours, and samples were processed for liquid β-galactosidase assay. (B) Effect of recA and sulA deletions on cell growth during dGTP starvation. Shown are the normalized viable counts (see Legend to Fig. 3) of the optA1 gpt (filled squares), optA1 gpt recA (open circles), and optA1 gpt sulA strains (open triangles) during Hx starvation initiated at time zero.