Table 1. Primers used for construction of chromosomal gene deletions.

Target gene	Pairs of primers (5′-3′)	Template
purR	ATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACGTGTAGGCTGGAGCTGCTTC TTAACGACGATAGTCGCGGAACGGGCCGTCAGCCACGGAGCGGCGTTCAACATATGAATATCCTCCTTAG	pKD3
recA	ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGTGTAGGCTGGAGCTGCTTC TTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGACATATGAATATCCTCCTTAG	pKD3
sulA	ATGTACACTTCAGGCTATGCACATCGTTCTTCGTCGTTCTCATCCGCAGCGTGTAGGCTGGAGCTGCTTC TTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCATATGAATATCCTCCTTAG	pKD3