Table 2. Primers specific to the origin (ori) and the terminus (ter) regions of the E. coli chromosome [49] used for ori/ter ratio determination using Quantitative PCR.
Region | Pairs of primers (5′-3′) |
ori | GAGAATATGGCGTACCAGCA AAGACGCAGGTATTTCGCTT |
ter | TCCTCGCTGTTTGTCATCTT GGTCTTGCTCGAATCCCTT |