Figure 6. [RNQ+] variants rely on putative Sis1 binding sites to different extents.

Propagation of s.d. high [RNQ+] is eliminated faster than s.d. low [RNQ+] when Rnq1-A3+A10 is expressed. Yeast cells propagating the indicated [RNQ+] variant and expressing either WT Rnq1 or Rnq1-A3+A10 were successively passaged three or six times after plasmid shuffle. Cell lysates were subjected to SDD-AGE and western blot using an αRnq1 antibody, and are representative of three independent experiments.