Figure 3. In vitro evaluation of the trypanocidal activity and cytotoxicity of H₂bdtc – (concentrations range from 125 µM to 0.24 µM in serial dilutions).

(A) Percentage of trypanocidal activity of free H₂bdtc and H₂bdtc loaded in SLNs against the Y strain of T. cruzi analyzed by quantifying viable parasites via microscopy after 24 h post-treatment. (B) Percentage of cytotoxicity of free H₂bdtc and H₂bdtc loaded in SLNs in spleen cells derived from mice after 24 h via propidium iodide treatment and FACS analysis. The mean + SEM is shown and is representative of three independent experiments (n = 2). Statistically significant differences compared with the control (BZN). Anova, Bonferroni post - test: ***p<0.001