Figure 5. axn and rbf mutations synergistically upregulate Hid expressing depending on deregulated TORC1 activity.

rbf mutation upregulated Hid protein and hid-lacz expression just anterior to MF (A and D), axn¹²⁷ did not change Hid protein or hid-lacz expression (B and E), while rbf axn¹²⁷ double mutations induced Hid protein or hid-lacz expression in a broad region anterior to MF region (C and F). hid mutation rescued rbf axn^S apoptosis (G–H). Quantification of C3 levels was shown in panel (I). rheb mutation blocked upregulation of Hid protein in rbf axn^S mutant clones (J–K′) and Hid-lacZ expression in rbf axn^EY mutant clones (L–M′). White arrowheads in J–M′ point to different levels of Hid (J–K′) or β-gal (L–M′) in rbf axn^S and rbf axn^S rbeb mutant clones. The yellow stars in L–M′ mark the background red channel signals at the lateral sides of the discs.