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. 2014 May 8;10(5):e1004297. doi: 10.1371/journal.pgen.1004297

Figure 1. RPM-1 binds to the PP2C phosphatase PPM-2.

Figure 1

(A) RPM-1::GFP was transgenically expressed in the neurons of C. elegans alone or in combination with FLAG::PPM-2. Coprecipitating RPM-1::GFP was detected with FLAG::PPM-2 (upper panel). Levels of FLAG::PPM-2 (middle blot) and RPM-1::GFP (lower blot) were determined by immunoprecipitation (IP). (B and C) At left are epifluorescent images of transgenic animals expressing GFP from the native ppm-2 promoter (Pppm-2::GFP). At right are schematic diagrams of the cells, nerve cords or regions of interest. Pppm-2::GFP expression was detected in (B) the nerve ring, and in (C) the dorsal and ventral nerve cords. (D and E) Shown are epifluorescent images of transgenic animals expressing both GFP from the native ppm-2 promoter (Pppm-2::GFP) and mCherry from a cell-specific promoter for the mechanosensory neurons (Pmec-7::mCherry). Expression of Pppm-2::GFP detected in (D) an ALM mechanosensory neuron (arrow) and (E) a PLM mechanosensory neuron (arrow). In all cases, multiple independently derived transgenic lines showed similar results, and images from a representative transgenic line are shown. Scale bars are 10 µm.