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. 2014 May 8;10(5):e1004297. doi: 10.1371/journal.pgen.1004297

Figure 7. ppm-2 regulates synapse formation by GABAergic motor neurons.

Figure 7

(A) Upper panel is a schematic diagram modified from Worm Atlas showing the GABAergic DD neurons that innervate the dorsal muscles (DD cell body and axon in purple, and presynaptic terminals shown in green). A transgene, juIs1 (Punc-25SNB-1::GFP), and epifluorescent microscopy was used to visualize the presynaptic terminals of the DD motor neurons for the indicated genotypes. Arrows note regions of the dorsal cord where presynaptic terminals are absent. Arrowheads highlight abnormal aggregation of presynaptic terminals. Scale bar is 10 µm. (B) Quantitation of synapse formation defects. Shown are averages for data collected from 3 or more independent experiments performed at 25°C in which 15–20 synchronized, young adult worms (16–20 hours post L4) were analyzed. Error bars represent the standard error of the mean, and significance was determined using an unpaired t-test. *p<0.05, ***p<0.001 and ns  =  not significant.