Skip to main content
. 2014 May 8;10(5):e1004116. doi: 10.1371/journal.ppat.1004116

Figure 2. HAT inhibition impairs Smp14 transcription and translation in S. mansoni adult worms.

Figure 2

Ten adult worm pairs were cultivated with increasing concentrations of the HAT inhibitor PU139 or DMSO as vehicle. PU139 concentrations between 0.01 and 20 µM were solubilized in 0.1% DMSO, and 50 µM PU139 was only soluble in 0.5% DMSO. (A) qRT-PCR analysis of Smp14 mRNA levels after a two-day incubation time. (B) Western blot analysis of total protein extract from a two-day worm pair culture using polyclonal antibodies against Smp14 (upper panels) and monoclonal antibodies against α-tubulin (lower panels) as loading control. (C) qRT-PCR of Smp14 after a longer period (four days) of PU139 (20 µM) treatment. (D) Smp14 protein levels at the fourth day of treatment with 20 µM PU139. Graphs are pooled from three independent experiments. Student's t-test was applied, with *p<0.05 and ***p<0.001. Western blot data are representative of three independent experiments.