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. 2014 May 8;10(5):e1004116. doi: 10.1371/journal.ppat.1004116

Figure 3. PU139 can reverse histone acetylation, keeping the Smp14 promoter in a repressed state.

Figure 3

Ten adult worm pairs were cultivated with 20 µM PU139 or vehicle for 48 h. (A) Western blot analysis of total worm extract. The levels of S. mansoni acetylated histone H3 and H4 are shown. (B–E) ChIP analysis of S. mansoni histone modifications at the Smp14 promoter. Fifty worm pairs were treated with 20 µM PU139 or vehicle at different time points and submitted to chromatin extraction. Chromatin was immunoprecipitated with antibodies directed against acetylated H3 and H4, RNA pol II (markers of transcriptionally active chromatin) and H3K27me3 (a marker of repressed chromatin). ChIP DNA (Smp14 promoter) was quantified by real-time PCR and normalized as a percentage of input DNA. Results are pooled from four independent experiments. Student's t-test was applied, with *p<0.05, ***p<0.001 and ****p<0.0001.