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. 2014 May 8;10(5):e1004116. doi: 10.1371/journal.ppat.1004116

Figure 4. HAT inhibition affects the normal development of S. mansoni eggs.

Figure 4

Ten adult worm pairs were cultivated with 20 µM PU139 or vehicle up to four days, and the number of eggs was counted on a daily basis (A). The estimated areas (length and width) of these same eggs were measured (B). Student's t-test was applied, with ***p<0.001. The morphology of the eggs was analyzed under optical microscopy. Scale bars: 10 µm (C). Adult worm pairs under the same treatment were fixed and stained with hydrochloric carmine for confocal laser scanning microscopy (CLSM) analysis. ne: normal egg; ae: abnormal egg; ot: ootype; vc: vitelline cells; u: uterus. The arrowhead in “ae” points to a fissure. tt: tegument tubercles. Scale bars: 20 µm (D).