Figure 6. HAT inhibition compromises the reproductive system of female worms.

Ten adult worm pairs were cultivated with 20 µM PU139 (panels b, d and f) or vehicle (panels a, c and e) for two days. The worms were fixed and stained with Certistain (Merck) for confocal laser scanning microscopy (CLSM) analysis. ne: normal egg; ae: abnormal egg; ot: ootype; ov: ovary; mo: mature oocytes; io: immature oocytes; vd: vitelline ducts; vc: vitelline cells; rs: receptaculum seminis. The PU139 treatment provoked several negative phenotypes: 1 - the morphology of the ovaries and oocyte maturation (panel b); 2 - the vitellaria size and the number of vitelline cells (panel d); 3 – The vitelline duct and ootype depleted of vitelline cells (panel f). Scale bars: 20 µm.