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. 2014 Apr 23;4:e28907. doi: 10.4161/mge.28907

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Copyright © 2014 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name mge-4-e28907-g2.jpg — Figure 2. Features of the snap-velcro model of L1 reverse transcription priming. (A) Reverse transcription priming only occurs if the DNA substrate is single-stranded. (B) Reverse transcription priming requires base-pairing between the L1 RNA (pink) poly(A) tail and the target-site DNA (green). The snap (bold green) corresponds to the last 4 nucleotides at the 3′ of the DNA primer. The velcro (light green) contains the 6 bases upstream of the snap. The snap is considered as closed if 4 nucleotides are T. The velcro is tightly fastened if the position-weighted T-density is superior to 0.5 (see ref. 28 for the detailed numerical model). The snap-velcro status predicts the efficiency of L1 reverse transcription priming (green arrow).